Klotho_Dosage, benefits & legal status
Also known as alpha-Klotho, a-Klotho, soluble Klotho, s-Klotho, KL
A real longevity protein, and a category where what is sold may not be it.
Common vial size is what vendors typically sell, not a recommendation. Enter your own vial in the calculator.
What is Klotho?
Plain language first. The technical label stays, but it never stands alone.Overview
It is a protein the body makes less of as you age, and mice given more of it live longer.
Alpha-Klotho is a large transmembrane protein whose extracellular portion is shed into the blood as soluble Klotho, acting as a circulating hormone with effects on kidney, brain and vascular aging.
What it's used for
A longevity protein with striking animal data, sold in vials and nasal sprays whose contents are frequently not the protein itself.
How does Klotho work?
What it does in the body, and where.Klotho was found by accident, in mice that aged fast because the gene was broken.
- 01The gene was identified in 1997Mice lacking it developed osteoporosis, atherosclerosis, infertility and died early. Mice overexpressing it lived longer.
- 02The membrane form is a co-receptor for FGF23That is its day job, regulating phosphate and vitamin D through the kidney.
- 03A soluble form is cleaved off and circulatesThis is the one the longevity interest is about, and blood levels fall from roughly the third decade of life onward.
- 04Getting more of it into a person is the unsolved partIt is a large protein with poor bioavailability, rapid clearance and difficult manufacturing, and it does not readily reach the brain. Every published human-facing effect is an association in blood levels, not a result of giving anyone the protein.
A schematic of the compound's mechanism, not a diagnostic image.
What else is it used for?
Each use below carries its own evidence level. Approved means regulators have cleared it for that purpose. Investigational means it is in trials. Early research means it is being studied and is not established.
- Extending lifespan and slowing aging markers, in miceEarly research
- Cognition and brain agingEarly research
- Kidney and cardiovascular protectionEarly research
- General longevity, as soldResearch only
The published record
Papers and trials about Klotho
57 papers tagged human · 30 not reviews or lab · 1 clinical trial publications
Furthest stage reached: Published trials only, 1 clinical trial publication, no phased trial registered
Counted from Europe PMC indexing, not read by a person; a human tag can land on reviews, commentary or lab papers; clinical trial publications is the narrower count.
At a glance
Four things to know about Klotho
Evidence
Category
Longevity and energy
Common vial
Varies
Half-life
No verified human figure
How much, and do you cycle it?
The range, and whether you take breaks.No established human dose, no established schedule. No established schedule No range
No human protocol exists. The mouse study most often cited dosed daily for ten weeks, which is a study design rather than a schedule for anyone.
No verified human figureNo figure
Described in the literature as rapidly cleared, which is one of the stated obstacles to using it as a therapy. No human figure has been verified here, and it would in any case depend on which construct is in the vial.
You can compare this against the whole library to see where it sits.
How do I dose and price it?
Enter your vial and your dose. Pick a mix. We show the draw and the cost.The vial holds a fixed amount of drug. Adding more water does not make more drug, it just spreads it thinner, so you draw a bigger number on the syringe for the same dose.
Choose your mix
Measuring the water1 mL = one full 100-unit syringe.
Klotho starts at $249.50 across 1 vendor.
3 listings, last read Oct 10, 2026. Shipping and card fees are not included.
How do you store it?
Before mixing, and after.Fridge after opening. Use within about 30 days.
Store sealed Klotho in the fridge. Once opened, keep it upright in the fridge and plan to finish it inside roughly 30 days. Keep it out of light and do not freeze the liquid.
What are the side effects and cautions?
Known cautions and warnings for this compound.Who should avoid it
- Any active cancer, since growth-factor signaling effects are uncharacterised
- Existing kidney disease, where Klotho and phosphate handling are tightly linked and the direction of effect is not established
- Pregnancy and breastfeeding
- Children
Stop and get help
- Swelling in the face, hands or feet
- New lumps or masses
- Spreading redness, heat or swelling at an injection site
- Signs of an allergic reaction such as face or throat swelling, hives, or trouble breathing
Common effects
- Unknown in humans
- No published human safety data
- Injection site reactions would be expected
Where this page says nothing is established, that means nobody has studied it, not that a compound is safe.
Is Klotho approved?
And where the numbers on this page come from.No. Klotho is not approved for any use, anywhere. It is sold labeled for laboratory research only, and there is no legal route to buy it for human use.
- Approval statusResearch only
- RouteUnder the skin, or as a nasal spray
- Checked24 August 2026
- DoseNo dose has been established in people.
- Vial sizeFrom what vendors typically list; not a recommendation.
- Sources
What has happened recently
Not approved anywhere, in any form. There is no approved recombinant Klotho protein, no approved gene therapy and no approved small-molecule Klotho activator, and no exogenous Klotho product has been validated in a human clinical trial. The evidence that draws people to it is genuine: transgenic overexpression extends mouse lifespan, and in humans, higher circulating levels are associated with lower mortality, better cognition and preserved kidney function. Association in blood levels is a different thing from a demonstrated effect of injecting a protein, and that gap is the whole story here.
You can compare this against the whole library, or read how to check a seller.
What else is like Klotho?
Others in Longevity and energy, side by side.If you're weighing Klotho, it's worth reading ALT-711, Orexin A, and PNC-28 in the same class.
A real longevity protein, and a category where what is sold may not be it.
- Dose
- No established human dose
- How often
- No established schedule
An anti-ageing molecule from the 1990s that broke sugar-protein crosslinks in rats and disappointed in people.
- Dose
- Research only
- How often
- No established pattern
A peptide studied for wakefulness and daytime energy.
- Dose
- Research only
- How often
- As studied
The lesser-known sibling of PNC-27, with the same cancer claims and the same missing human trials.
- Dose
- Research only
- How often
- Varies
Guides for this compound
- How to Reconstitute a Peptide
Mix peptide powder with bacteriostatic water, step by step.
- How to Read an Insulin Syringe
Read the units, convert to millilitres, and draw the exact dose.
- mcg vs mg: The Peptide Dosing Mistake to Avoid
Your vial says mg and your protocol says mcg. Here is the conversion and how to check it.
- How to Read a Peptide COA
What the purity number does and does not tell you, and how to spot an edited report.
- How to Vet a Peptide Vendor
Independent COAs, HPLC purity, and the red flags that mean walk away.
Sources
- Anti-inflammatory actions of soluble Klotho in brain ageing, PMC(2025)Gene identified in 1997; loss produces accelerated aging phenotypes; two major isoforms.
- Serum soluble alpha-Klotho and cardiovascular disease, PMC(2022)Transgenic overexpression extends mouse lifespan by 20 to 30 percent; human investigation remains limited and associative.
Beyond these, 118 records are indexed. Browse the records